Experimental Investigation of Gas Degradation by Methanotrophs at Different Air Flow-rates

Hong Yu, Tao Feng, Ruilin Zhang, Jie Liu


A technical approach based on microbial technology is proposed to help resolve the problems caused by explosive gases
in coal mines. The proposed technique uses methanotrophic bacteria to oxidize methane. In laboratory experiments, the
oxidation eect of hanging nets impregnated with liquid containing methanotrophic bacteria was investigated at dierent
air flow-rates. The experimental results showed that the volume of gas degraded and the gradient of degradation both
increased as the gas concentration increased at constant air flow-rates. At fixed gas concentrations, the volume of
degraded gas increased with increasing flow-rates of air at low flow-rates. However, the volume of degraded gas slightly
decreased with increasing flow-rates of air at high flow-rates. These experimental results provide a theoretical basis for
the treatment of explosive gases during exploration for natural gas and to treat potentially dangerous concentrations of
gas in gobs, caves and upper corners of mineshafts. They will also be of great practical significance in coal mining.

Full Text:



Marian Marschalko, Martin Bednárik, Işık Yilmaz, et al., Evaluation of subsidence due to underground coal mining: an example from the czech republic, Bulletin of Engineering Geology and the Environment 7(1)(2012)105-111.

Karancan C. O., Ruiz F. A., Cote M., et al., Coal mine methane: a review of capture and utilization practices with benefits to mining safety and to greenhouse gas reduction, International Journal of Coal Geology 86(2-3)(2011)121-156.

A.Yu.Kallistova, M.V.Kevbrina, V.K.Nekrasova, et al., Methane oxidation in landfill cover soil, Microbiology 74 (5)(2005)608-614.

G. G. Sakantsev, M. G. Sakantsev, V. I. Cheskidov, et al., Improvement of deep-level mining systems based on optimization of accessing and open pit mine parameters, Journal of Mining Science 50(4)(2014)714-718.

Svetlana Kotelnikova, Microbial production and oxidation of methane in deep subsurface, Earth-Science Reviews 58(3-4)(2002)367-395.

K. T. Win, R. Nonaka, A. T. Win, et al., Comparison of methanotrophic bacteria, methane oxidation activity, and methane emission in rice fields fertilized with anaerobically digested slurry between a fodder rice and a normal rice variety, Paddy and Water Environment 10(4)(2012)281-289.

Santosh Ranjan Mohanty, Bharati Kollah, Vineet K. Sharma, et al., Methane oxidation and methane driven redox process during sequential reduction of a flooded soil ecosystem, Annals of Microbiology 64(1)(2014)65-74.

Karakurt I, Aydin G, Aydiner K., Mine ventilation air methane as a sustainable energy source, Renewable and Sustainable Energy Reviews 15(2)(2011)1042-1049.

Cheng Yuanping, Liu Hongyang, Guo Pinkun, et al., A theoretical model and evolution characteristic of mining-enhanced permeability in deeper gassy coal seam, Journal of China Coal Society 39(8)(2014)1650-1658.

Wang Lu, Jin Longzhe, Chen Dongke, Research prospect of applying microorganism in controlling coal gas, China Safety Science Journal 15(10)(2005)97-99.

Ding Xia, Peng Xiaojue, Min Hang, et al., Molecular mechanism of stress resistance in methanothermobacter thermoautotrophicus, Chin J Appl Environ 17(3)(2011)307-310.

Zhang Ruilin, Cai Chuanhui, Wang Zhenjiang, The preliminary experiment research on gas degradation by microorganism under high-pressure and poor-oxygen conditions, China Mining Magazine 23(11)(2014)132-135.

Marjalizo-Cerrato P.J., Tejero-Manzanares J., Mata-Cabrera F., et al., Intervention, Search And Rescue Equipment Within Confined Spaces, DYNA 88(2)(2013)216-225.


  • There are currently no refbacks.